极谱免疫法测定抗血清中溶血素的活性

Determination of Hemolysin Activity in Antiserum by Polarographic Immunoassay

本文报道一种测定溶血素活性的新方法。利用免疫溶血反应释放出的血红蛋白来催化邻苯二胺和H2O2的反应，通过酶促产物2，2＇-二氨基偶氮苯的极谱检测来确定溶血素的活性。测定范围为0．02～4.50U／mL，检测下限为0．018U／mL；一次可分析25份血样，已用于抗血清样品中溶血素活性的测定。

A new method for determination of hemolysin activity in antiserum by polarographic immunoassay was reported in this paper. This method is based on the reaction between o-phenylenediamine and hydrogen peroxide catalyzed by hemoglobin released from cells in immune hemolysis, producing an electroactive compound 2, 2' -diaminoazobezene which is determined by linear-sweep polarography.The linear range is 0. 02 ~ 4. 5 U/mL with a detection limit of 0. 018 U/mL.The proposed method has been applied to the determination of hemolysin activity in antiserum samples.