摘要
本文采用狂犬病毒CTN-1和4aC株,经Vero细胞传代适应后,以Vero细胞为培养基质,建立了狂犬病毒蚀斑试验和蚀斑减少试验的方法。目前已将此方法应用于病毒鉴定、病毒克隆、病毒滴定以及抗狂犬血清的检测,并取得了较好的结果。
We explored rabies virus adapted to Vero cells making plaque formation promptly.The plaque could be all exposed and easily seen under microscope on 4th day. It was shown that plaque method was more sensitive than mice(i.c) method on the titer of virus and assay neutralizing antibody.Up to now, the plaque formation has been applied to virus cloning, virus identify, virus titration,evaluation of serum-antibody content by the virus neutralization technique,providing an important assay method for the study and preparation for Purified Vero Rabies Vaccine(PVRV).
出处
《病毒学杂志》
CSCD
1990年第2期135-141,共7页
关键词
狂犬病毒
蚀斑
Rabies virus
Plaque method
