摘要
基于青霉素在青霉素酶的作用下会水解生成青霉素酸,降低了系统的pH值,一个青霉素流动注射分析系统在我们实验室建立起来了。大量的实验结果表明,青霉素浓度与[H ̄+]输出峰面积成正比,与[H ̄_]输出峰高成非线性关系,青霉素浓度的峰面积计算法比峰高计算法具有更高的准确性。
ased on the enzymatic hydrolysis ofpenicillin into penicilloic acid which reducesthe pH value in the injected sample with im-mobilized penicillinase on silica gel,a flow in-jection analysis(FIA)system was developedfor the measurement of penicillin. All the ex-perimental results show that the penicillinconcentration is proportional to the area out-put signal of hydrogen ion concentration , butnot to the peak height.The calculated resultsbased on the area output signal of hydrogenion concentration are much rnore precise andaccurate than those based on the peak height.
出处
《分析试验室》
CAS
CSCD
北大核心
1996年第4期8-11,共4页
Chinese Journal of Analysis Laboratory
基金
国家自然科学基金
国家教委留学回国人员基金
广东省自然科学基金
关键词
流动注射分析
酶反应器
青霉素
FIA systemsenzymatic reac-tor
measurement of penicillin