测定青霉素浓度的流动注射分析系统及其特性的研究

A Flow Injection Analysis System for theMeasurement of Pemcillin and Its Character-istics

基于青霉素在青霉素酶的作用下会水解生成青霉素酸，降低了系统的ｐＨ值，一个青霉素流动注射分析系统在我们实验室建立起来了。大量的实验结果表明，青霉素浓度与［Ｈ￣＋］输出峰面积成正比，与［Ｈ￣＿］输出峰高成非线性关系，青霉素浓度的峰面积计算法比峰高计算法具有更高的准确性。

ased on the enzymatic hydrolysis ofpenicillin into penicilloic acid which reducesthe pH value in the injected sample with im-mobilized penicillinase on silica gel,a flow in-jection analysis(FIA)system was developedfor the measurement of penicillin. All the ex-perimental results show that the penicillinconcentration is proportional to the area out-put signal of hydrogen ion concentration , butnot to the peak height.The calculated resultsbased on the area output signal of hydrogenion concentration are much rnore precise andaccurate than those based on the peak height.