摘要
在同一块薄层板上多相二维分离30余种氨基酸、40余种非挥发性有机酸和20余种碱基。核苷类化合物。应用一对新的展开剂,用紫外灯-吖啶试剂-镉茚三酮试剂组合显色定位分析,改良了尿液非挥发性有机酸的溶剂萃取。尿液中此三类化合物提取物的多相二维色谱分离效果良好。
Over 30 amino acids, 40 organic acids and 20 nucleosides and related compounds have been simultaneously separated by two-dimensional thin layer chromatography on a plate. The method of solvent extraction for the non-volatile organic acids in urine was improved. And the location of the separated compound spots was performed with a multiple procedures: Viewing the absorption spots in UV light (254nm)---colouration in acridine reagents for most organic acids and ninhydrin-cadmium acetate for amino acids.
出处
《色谱》
CAS
CSCD
北大核心
1996年第4期259-263,共5页
Chinese Journal of Chromatography
关键词
二维薄层色谱法
氨基酸
有机酸
碱基核苷类
尿
two-dimensional thin-layer chromatography, amino acids, organic acids, nucleosides, urine