固相萃取结合毛细管气相色谱法分析血浆中5种氨基甲酸酯

Simultaneous Determination of 5 Carbamates in Human Plasma by Using Solid-Phase Extraction and Capillary Gas Chromatography

报道了用Ｘ－５树脂固相萃取分离纯化、气相色谱法（ＧＣ）定性和定量同时测定血浆中５种氨基甲酸酯杀虫剂的方法。含药物的血浆可直接用萃取柱处理，用甲醇－乙酸乙酯作为洗脱剂。不同药物的回收率在８０．０％～９２．５％之间，最低检测浓度为１ｍｇ／Ｌ，检测灵敏度及线性范围（０～４０ｍｇ／Ｌ）适合临床中毒急救分析的需要。

A capillary gas chromatographic method for identification and simultaneous quantitation of 5 carbamate drugs-metolcarb, isoprocarb, bassa, carbofuran and carbaryl, in human plasma is described. The method employed X-5 macro-reticular resin as adsorbent for the separation and purification of the carbamates from plasma. The extraction efficiency of the home-made solid phase extraction column and three other commercial columns with different packing materials, and the sample evaporation approaches are compared and discussed in detail. The recoveries of drugs were examined by comparing the peak area of each carbamate spiked plasma with that obtained after injection of a known amount of standard. Identification of each carbamate was achieved by retention time. Detection limits was 1μg/mL in 0.5mL plasma. Recoveries were in the range of 80. 0%-92. 5%. Precision[within day:RSD 6. 3%-6.8%(n = 6),day-to-day:RSD 6. 6%-10. 8%(n=4)] and linearity(r>0. 99 in the range of 0-40mg/L) are satisfactory for clinically toxicological applications.