液相色谱法测定血浆中三环类抗忧郁药物

High Performance Liquid Chromatographic Analysis of Tricyclic Antidepressants in Blood Samples

用高效液相色谱法测定了血浆中４种三环类抗忧郁药物：卡马西平、氯氮平、多虑平和阿米替林。方法采用Ｃ_（１８）色谱柱分离，二极管阵列检测器检测，流动相为甲醇：０．５ｍｏｌ／Ｌ三乙胺－０．５ｍｏｌ／Ｌ冰醋酸的水溶液（９０：１０，Ｖ／Ｖ；ｐＨ７．５）。标准曲线的线性范围为０～１０ｍｇ／Ｌ，相关系数在０．９９以上。方法的最小检出浓度为１０～４０μｇ／Ｌ血浆。血中药物经溶剂萃取后，药物的回收率为７３．６７％～９８．２４％，血中杂质不影响药物的检测。方法简便、快速，适用于临床中毒样品的分析。

Carbamazepine, clozapine, doxepine and amitriptyline are tricylic antidepressants used in the clinic. In the paper, a reversed-phase HPLC method is described for analysis of tricyclic antidepressants in human plasma.The method used a Spherisorb C_(18)column (150mm×2. 0mm i. d. , 5μm) and photodiode array detector. These drugs were identified by their spectrum characterestics and retention times and quantitatively determined by their peak areas. The mobile phase consisted of methanol-10% water solution containing 0. 5mol/L triethylamine and 0. 5mol/L acetic acid adjusted to pH 7. 5. Plasma samples were extracted with diethyl ether. The linear range of the calibration curves were 1-10 mg/L and correlation coefficients were all over 0. 99. The average recoveries of drugs ranged 73. 67%-98. 24%. The relative standard deviations of this method were 3. 65%-5. 32%. The lowest limits of detection were 10-40μg/L plasma. This method is simple ,rapid ,sensitive and of excellent precision.