摘要
采用高效液相色谱法（ＨＰＬＣ）测定了中成药伸筋丹中士的宁含量。色谱条件：氰基柱并加同种类预柱；流动相：甲醇－水－乙酸－三乙胺（９８００：１５５：３０：１５）；ｐＨ６．７；流速：１．０ｍＬ／ｍｉｎ；紫外检测，波长２５４ｎｍ；在４～２０ｍｇ／Ｌ浓度范围内，士的宁的峰面积Ａ与浓度Ｃ间的回归方程为：Ａ＝－７．０１×１０~３＋９．０８×１０~５Ｃ（ｒ＝０．９９９９）；外标法定量，方法精密度ＲＳＤ＝１．９１％（ｎ＝６）。
An HPLC method was developed for the determination of strychnine contained in Shenjin Dan.The analysis was carried out by using ultrasphere￣(TM)-cyano column (5μm, 4. 6mm×150mm),the mobile phase consists of methanol-water-acetic acid-triethylamine (9800:155:30:15). Detection was done spectrophotometrically at 254nm, calibration graphs were rectilinear over the concentration ranges of 4-20mg/L for strychnine (r=0. 999 9). The average recovery of strychnine was 98. 9%. The precision for sample determination was RSD=1. 91%.
出处
《色谱》
CAS
CSCD
北大核心
1996年第4期321-322,共2页
Chinese Journal of Chromatography
关键词
高效液相色谱法
士的宁
伸筋丹
high performance liquid chromatography, strychnine, Shenjin Dan
