钌（Ⅱ）－联吡啶－巴比妥酸－Ce（Ⅳ）化学发光体系测定巴比妥酸的研究

Chemiluminescent Determination of Barbituric Acid With Ru(bipy) 2+ 3 Barbituric Acid Ce(Ⅳ) System

利用巴比妥酸对Ｃｅ（Ⅳ）氧化钌（Ⅱ）－联吡啶化学发光反应的增强作用，建立了痕量巴比妥酸的自动脉冲注射化学发光测定法方法的检出限为１０×１０－９ｇ／ｍＬ，线性范围为５０×１０－８－６．２×１０－５ｇ／ｍＬ巴比妥酸的衍生物如巴比妥。

The reaction of Ru(bipy) 2+ 3 with Ce(Ⅳ) produces chemiluminescence in aquous soluion. Barbituric acid can enhance the light emission. Chemiluminescence intensity was linearly related to the concentration of barbituric acid over the range of 5.0×10 -8 -6.2×10 -5 g/mL. The detection limit was 1.0×10 -9 g/mL. The method was applied successfully to determine barbituric acid in synthesized samples.