Raji细胞培养中HSV持续感染模型的建立及免疫因素(TNF IFNs)对它的影响

The Establishment of Persistent Infection of Herpes Simplex Virus in Raji Cell and the Effects of TNF, IFNs and Antiserum to It

本文观察HSV持续感染Raji细胞培养物150天。发现整个感染过程似呵分为两个阶段:急性期(前30天)和稳定期(30天以后)。在急性期上清液中HSV滴度达10^(6.2)TCID_(50)/ml.病毒抗原阳性细胞达21％,死细胞达42％;在稳定期病毒和细胞处于相对平衡状态,上清液中IISV滴度在10^(3.5-4.2)TCID_(50)/ml,病毒抗原阳性细胞约占5—10％,感染细胞与对照细胞在生长特性上无明显差异。用rIFNs、rlL-2和TNF处理稳定期的细胞培养物,发现TNF和rlFNa能明显抑制HSV的复制,rIENy作用较弱,去除上述因子5天后又恢复到处理前水平。rlL-2无明显作用。用HSV抗体处理上述细胞培养物上清液中病毒和病毒抗原阳性细胞都消失,且在去除抗体后连续观察50天仍未出现。本实验为体外研究HSV持续感染提供了一个有用的模型。

Persistent infection of herpes simplex virus(HSV) had been maintained in human B lymphoblastoid(Raji) cells for 150 days. The whole course of the persistent infection can be divided into two phases: acute(within 30 days p. i.) and persistent stable(30 days later p.i.). In the acute phase,the virus in the supernatent reached 10^(6·2)TCID_(50)/ml, HSV antigen positive cells(HSV Ag^+ cell) detected by immunofluorescence reached 21% and the percentage of dead cells reached 42%. In the stable phase, the virus and Raji cells became relatively balanced: virus in the supernatent ranged 10^(3·5-4·2) TCID50/ml,HSV Ag^+ cell ranged 5—10% and the dead cell was less 5%(the same as controls). Treating the cell culture with recombinant interferons(rIFNα,rIFNγ),recombinant interleukin 2(rIL-2) and tumor necrosis factor(TNF) respectively,we found that TNF and rIFNα inhibited the virus replication markedly while so did rIFNγ but weakly and rIL-2 had no effects Removing the factors 5 days later, the virus and HSV Ag^+ cells gradually recoved as controls. Treating the cultures with anti-HSV serum, the virus and Ag^+ cells were diminished and undetectable. After removing the antibody,the virus and its Ag^+ cells no longer repeated and so did continuously for 50 days,These studies provided an useful model for research on HSV persistent infection in vitro in many aspects.