摘要
在镀银电极的压电石英晶体表面涂覆甲基丙烯酸甲酯-甲基丙烯酸羟乙酯共聚物涂层,经CNBr活化后固定抗体,用石英晶体微天平技术监测IgM抗原在压电石英晶体上的吸附与解吸过程,研究了免疫反应体系IgM-抗人IgM的反应动力学.结果表明:IgM在压电石英晶体上的键合行为很好地符合Langmuir吸附方程,免疫反应的结合常数Kass为7.10×10 ̄7(mol/L) ̄(-1).在抗原过量情况下为一级反应。计算了IgM免疫反应的活化能Ea和不同温度下的反应速率常数kf.
The adsorption and desorption of Immunogobulin M(IgM) antigen on the ecrystal modified with anti-IgM antibody have been continuously monitored and the immunoreaction kinetics of IgM-antigen with the anti-IgM antibody has been investigated by using the quartzcrystal microbalance methodology.Immobilization of the anti-IgM antibody to the crystal's surface was accomplished via a CNBr-activated copolymer coating of hydroxyethyl-methacrylate(HEMA) and methyl-methacrylate (MMA),The results indicated that the binding behavior of IgM on the crystal's surface was fitted for the Langmuir adsorption equation quite well.The association constant of the immunoreaction(Kass)calculated from this equation was7.10×107(mol/L)(-1).The kinetic equation of this reaction under the condition of excessive IgM antigen was derived.This equation was exactly fitted for the experimental results.The reaction rate constants (kf) at different temperatures and the Arrhenius activation energy(Ea) of the immunoreaction in this experimental system were calculated. It was inferred that the activation energy of this immunoreaction was mainly used to overcome the hydrousenergy of the molecules of antigen and antibody.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
1996年第7期1025-1029,共5页
Chemical Journal of Chinese Universities
基金
国家教育委员会博士学科点基金
关键词
石英晶体微天平
免疫反应动力学
免疫球蛋白M
Quartz crystal microbalance,IgM immunoreaction,Immunoreaction kinetics,Activation energy of immunoreaction