肠道传播非甲非乙肝炎在恒河猴中的传代研究

Study on Propagation of Enterically Transmitted Non-A,Non-B Hepatitis Agent in Rhesus Monkeys

用ET-NANBH感染的两只猴(R5和R6)含病毒颗粒的粪便悬液和肝组织悬液分别接种7只和4只恒河猴,分别有5只猴和4只猴在攻毒后20—49天内ALT开始升高,持续时间为7—10天,肝组织学的特征性改变为肝细胞的嗜酸性变和嗜酸小体的形成。在猴ALT升高前2—3天和升高后一周内均检查到大量的27—34nm的病毒样颗粒,这些颗粒只与ET-NANBH病人血清、黑猩猩和猴感染后急性期和恢复期血清发生特异性聚集。在猴感染后血清中未检出抗-HAV、抗-HAV-IgM、HBsAg和抗-HBe-IgM。结果提示:恒河猴是研究ET-NANBH较适宜的动物模型;病人和猴粪便中的27—34nm的病毒样颗粒是ET-NANBH的病原因子。

Significant alanine aminotransferase (ALT)elevation was observed in five of seven and all of four Rhesus monkeys (Maeaca mulata) between 20 and 49 days after intravenous inoculation of pool of stool suspensions and crude liver homogenate respectively that were derived from two monkeys (R5 and R6) infected with stool suspensions obtained from epidemiologically and serologically defined case of enterically transmitted non-A,non-B hepatitis(ET-NANBH) originating in Xinjiang, China, Charateristic histopathologic changes were acidophilic degeneration of the liver cell and sporadic acidophilic bodies, 27-to 34- nm virus-like particles were identified in stool specimens from a Chinese patient with ET-NANBH and experimentally infectd monkeys with the first and second passages of the disease. These particles were specifically aggregated by antibody contained in acute-phase sera from patients and acute-and convalescent-phase sera from ET-NANBH infected chimpanzee and monkeys. These findings indicated that Rhesus monkeys are suitable experimental animal models for studies of ET-NANBH; the liver was the site of virus replication; the 27- to 34- nm virus-like particles found in infected human and primate stool specimens may be etiological agent of ET-NANBH.