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Cloning and Characterization of a Novel cDNA Encoding Late Embryogenesis-Abundant Protein 5 Like (LEA-5) Gene from Cara Cara Navel Orange Fruit (Citrus sinensis Osbeck)

Cloning and Characterization of a Novel cDNA Encoding Late Embryogenesis-Abundant Protein 5 Like (LEA-5) Gene from Cara Cara Navel Orange Fruit (Citrus sinensis Osbeck)
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摘要 LEA5 gene was postulated related with both stress and hormone responses. In an attempt to find genes exclusively expressed during fruit ripening of Cara Cara navel orange, a novel cDNA clone encoding late embryogenesis-abundant protein 5 like gene (CitLEA5-1) was obtained. It was 582 bp in length, containing 97 deduced amino acids. Compared with the stress-induced LEA5 from leaves of Citrus sinensis, CitLEA5-1 had a shorter 3′ untranslated region (UTR). Semi-quantitative RT-PCR analysis revealed that CitLEA5-1 was transcriptional regulated during fruit ripening of Cara Cara navel orange. LEA5 gene was postulated related with both stress and hormone responses. In an attempt to find genes exclusively expressed during fruit ripening of Cara Cara navel orange, a novel cDNA clone encoding late embryogenesis-abundant protein 5 like gene (CitLEA5-1) was obtained. It was 582 bp in length, containing 97 deduced amino acids. Compared with the stress-induced LEA5 from leaves of Citrus sinensis, CitLEA5-1 had a shorter 3′ untranslated region (UTR). Semi-quantitative RT-PCR analysis revealed that CitLEA5-1 was transcriptional regulated during fruit ripening of Cara Cara navel orange.
出处 《Agricultural Sciences in China》 CAS CSCD 2006年第4期272-276,共5页 中国农业科学(英文版)
基金 the National Natural Science Foundation of China (30300241, 30471201).
关键词 CITRUS CLONING CHARACTERIZATION late embryogenesis-abundant protein Citrus, cloning, characterization, late embryogenesis-abundant protein
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参考文献4

  • 1Neng-guo Tao,Juan Xu,Yun-jiang Cheng,Xiu-xin Deng.Lycopene-ε-Cyclase Pre-mRNA is Alternatively Spliced in Cara Cara Navel Orange (Citrus sinensis Osbeck)[J].Biotechnology Letters.2005(11)
  • 2Nengguo Tao,Yunjiang Cheng,Juan Xu,Qiang Xu,Xiuxin Deng.An effective protocol for the isolation of RNA from the pulp of ripening citrus fruits[J].Plant Molecular Biology Reporter.2004(3)
  • 3Stephen Jackson,Jordi Gascón,Esther Carrera,Elena Monte,Salomé Prat.Cloning and expression analysis of a gene that shows developmental regulation upon tuberization in potato[J].Plant Molecular Biology.1997(1)
  • 4Dorit Naot,Gozal Ben-Hayyim,Yuval Eshdat,Doron Holland.Drought, heat and salt stress induce the expression of a citrus homologue of an atypical late-embryogenesis Lea5 gene[J].Plant Molecular Biology.1995(3)

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