肝病患者肝细胞内HBV DNA原位杂交研究——形态、分布及意义

Morphology, Distribution and Its Signification of Intrahepatic HBV DNA in Liver Disease: A Study by in Situ Hydridization

应用生物素标记HBV DNA(乙肝病毒脱氧核糖核酸)作探针,对129例肝病患者肝组织作原位杂交研究。发现HBV DNA主要存在肝细胞浆内,可分为胞浆致密型、疏松型和包涵体型。HBV DNA阳性肝细胞在肝实质中分为三种型:小叶型、局灶型与散在点状分布。HBV DNA在慢性活动型肝炎中检出率最高(81％),显著高于肝硬化,慢性小叶型肝炎、急性肝炎及原发性肝癌组。乙肝复制指标阳性患者肝细胞内HBV DNA检出率明显高于非复制组;并观察到HBV DNA阳性肝细胞与肝细胞坏死灶关系密切,多数紧紧毗邻肝细胞坏死灶/或和位于坏死灶中间,尤以局灶型分布的HBV DNA阳性肝细胞为显著。

A biotin-labeled DNA probe specific for hepatitis B virus(HBV)nucleotide sequences was hybridized in situ to liver tissue of 129 cases with liver disease. It was found that HBVDNA was predominantely visualized in cytoplasm of hepatocytes in three ways,cytoplasmic compact pattern,discrete pattern abd inclusion pattern. Its distribution in parenchyma within the section of specimens may be defined as lobular, focal and spotty. The detection of intrahepatic HBVDNA depanded on two factors at least in present study, 1: liver disease activity; as chronic active hepatitis(CAH)group had a significant higher prevalence (81%), compared to eirrhosis; chronic lobular hepatitis (CLH)acute hepatitis and hepatocellular carcinoma(HCC)groups: Ⅱ HBV infection status: HBVDNA in HBeAg positive/or intrahepatic HBcAg positive patients more easily detected than the single HBsAg positive or anti-HBc cases. The interesting finding that hepatocytes expressing HBVDNA hpatocytes, particularly as focal distribution were closedly related to hepatic necrostic sites suggests that HBV replication may occur in conjunction with hepatic necrosis