摘要
将行为学实验与免疫组化LSAB技术结合,观察了大鼠在佐剂性关节炎发生发展中及针刺治疗时,脊髓背角降钙素基因相关肽(CGRP)和P物质(SP)的动态变化。结果表明,正常大鼠CGRP主要分布于脊髓背角Ⅰ、Ⅱ、Ⅴ、Ⅹ层和利骚厄氏束,SP主要分布于脊髓背角浅层。注入佐剂后第2天形成急性关节炎时,背角浅层和灰质后联合中CGRP免疫活性物质(CGRP-LI)和SP免疫活性物质(SP-LI)有所增加;第14天发展为多发性关节炎时进一步明显增加,而且在灰质后联合中可见CGRP-LI长纤维;第28天后随佐剂性关节炎的逐渐恢复而恢复。电针双侧环跳穴可减轻佐剂性关节炎大鼠的自发痛和痛觉过敏,并可预防多发性关节炎的发生,同时可见CGRP-LI和SP-LI增加的反应减弱。结果提示。
Dynamic changes of CGRP and SP LI in dorsal horn at L5 ̄S1 in arthritic rats untreated and treated by EA at Huantiao points were analyzed with labelled streptavidin biotin (LSAB) immunohistochemical method on day 2, 7, 14, 21 and 28 after the injection of Freund′s complete adjuvant (FCA, 0.5 mg Mycobacterium tuberculosis per rat) into the right ankle. In the normal rats (n =5 or 6), CGRP LI were mainly present in laminae Ⅰ,Ⅱ,Ⅴ,Ⅹ and Lissauer′s tract of the spinal cord; SP LI were mainly present in the superficial layers of the dorsal horn. After the adjuvant injection both CGRP and SP LI increased corresponding to the development of arthritis. On day 2, CGRP LI was moderately increased in laminae Ⅰ,Ⅱ and the posterior commissure of the spinal cord, while SP was obviously increased in the superficial layers after the rats underwent acute arthritis. On day 14, CGRP LI markedly enhanced in the above regions with the development of polyarthritis. Sometimes many intensely stained long fibers could be seen in the posterior commissure. And then the arthritis gradually alleviated and recovered on day 28. In the arthritic rats (n =5 or 6), EA at points could alleviate hyperalgesia and persisting pain, and prevent the rats suffering from polyarthritis, meanwhile the increase of CGRP and SP LI was reduced. These results suggested that the dynamic changes of CGRP and SP in the superficial layers of dorsal horn are closely associated with the development of adjuvant arthritis.
出处
《中国兽医学报》
CAS
CSCD
1996年第5期417-423,共7页
Chinese Journal of Veterinary Science
基金
国家自然科学基金