MDVgB对鸡白细胞的粘着作用及其抗病毒免疫原性

Adhesion of the Recombinant Glycoprotein B of Marek′s Disease Virus to White Blood Cells and Its Immunogenicity against Infection

用抗马立克氏病病毒囊膜糖蛋白Ｂ（ＭＤＶｇＢ）的单抗ＩＡＮ８６作间接免疫荧光试验表明，在昆虫细胞中表达的重组ｇＢ基因产物能识别和粘着于鸡的部分淋巴细胞和巨噬细胞表面。抗重组ｇＢ的鸡血清在鸡成纤维细胞培养上对ＭＤＶ的中和效价为１∶８～１∶１６。经用能表达ＭＤＶｇＢ基因的重组杆状病毒感染的昆虫Ｓｆ９细胞免疫的鸡，能抵抗ＭＤＶ强毒株引起的急性死亡。

The adhesion of the recombinant glycoprotein B (r gB) of Marek's disease virus (MDV) expressed in insect cells to cell surface was demonstrated in a part of chicken peripheral blood white cells or macrophages from the ascitic fluids (about 2% 5% of cells) in the indirect fluorescence antibody test with monoclonal antibody IAN86 against MDVgB. The chicken sera against r gB of MDV were able to neutralize MDV in chicken fibroblast cell culture, the neutralizing titers were about 1∶8 1∶16. The chickens immunized with gB expressing recombinant baculovirus infected Sf9 cell lysates were protected from acute death after challenge with the virulent MDV ( P <0 01). But the immunization with r gB did not cause significant differences in the late mortalities and oncogenic rates between the immunized and control chickens.