摘要
用直接酶标和直接免疫荧光法在120例HBsAg阳性肝组织中检测HDAg,发现5例阳性(4.2％),其中3例活动性肝硬化,2例慢性中型活动性肝炎。另设立相对应的单纯乙型肝炎作为对照,通过光镜、电镜、免疫组化的方法,重点探讨比较丁型肝炎与单纯乙型肝炎二者肝组织学的形态变化。丁型肝炎组肝细胞灶状坏死,桥接坏死,融合性坏死的程度比单纯乙型肝炎组重,丁型肝炎组较单纯乙型肝炎组常见的组织学改变是肝细胞呈灶状微小空泡的脂肪变性及灶状嗜酸性变性。HDAg阳性肝细胞呈疏松化、核固缩,其周围未见淋巴细胞浸润。
To explore the histopathological features in chronic hepatitis D, 5 out of 120 cases of HBsAg-positive liver samples were found to be intrahepatitic HDAg-positive by direct immunof luorescence and immunoperoxidase assays. Among 5 cases, 3 had active cirrhosis, 2 cases with chronic active hepatitis. Wellmatched cases with chronic hepatitis B were used as control, Emphasis was put on similarity and difference in histopathological findings between hepatitis D and hepatitis B by light and electron microscopy and immunohistochemistry. In general, focal, bridging and confluent necrosis in hepatitis D resembled that of chronic hepatitis B, but to more severe degree. Characteristic microvesicular fatty degeneration and acidophilic degeneration in chronic hepatitis B which were less conspicuous were frequently present in hepatitis D. In addition, hepatocytes containing HDAg may undergo ballooning and karyopyknosis without peri-infiltration of lymphocytes.
出处
《病毒学杂志》
CSCD
1990年第4期352-356,共5页
