应用单克隆抗体的免疫组织化学法研究雏鸭体内鸭瘟病毒的分布

Indirect enzymatic detection to the duck enteritis virus with the monoclonal antibody

本实验应用了免疫组织化学的单克隆抗体间接酶标染色法,对人工感染鸭瘟病毒雏鸭的组织切片进行染色观察,旨在研究病毒在鸭体内分布,对其进行定位。研究结果显示,鸭的心脏、肝脏、脾、胸腺、肠、法氏囊、胰、肺、肾等组织的细胞浆内均出现了染色的特异阳性反应物。结果表明,鸭瘟病毒广泛分布于感染雏鸭的各种组织器官,并造成一定的组织病理变化。

The tissue sections from ducks which have been inoculated with duck enteritis virus （DEV） were stained by indirect enzyme monoclonal antibody immunoassay. The purpose was to detect the DEV distribution in the duck body. The results show that, the DEV antigen was detected in the heart, liver 、 spleen 、 thymus, intestine 、bursa、pancreas 、lung and kidney. The data show that the virus can infect many organs in duck body and produce pathologic changes in the organs.