两种前S蛋白与HBV复制关系的初步研究

Comparison of Two Pre-s Proteins in Hepatocytes with HBV Replication Status by in situ HBV DNA Hybridization Assay

乙型肝炎病毒（HBV）前S基因及其产物的发现有助于认识HBV复制规律。一些研究提示:前S1蛋白是完整病毒颗粒表面的必要成分,其存在与HBV复制密切相关,前S1检测能敏感和特异地反映HBV复制状态。另一些研究则认为:前S2蛋白含有人多聚白蛋白的结合位点,并提出前S2蛋白为HBV复制的新标志。为探讨HBV复制与两种前S蛋白之间的联系,本研究应用原位分子杂交分析一组慢性肝炎患者肝细胞内HBV DNA,同时检测肝细胞内HBsAg、HBcAg与两种前S蛋白,综合分析两种前S蛋白在HBV复制中的地位和作用。

To compare relationship between two pre-s proteins and HBV replication HBV DNA in situ hybridization was applied to a group of patients with chronic hepatitis by bio-probe, in conjunction of detection of intrahepatic HBsAg, HBcAg, pre-s1 and pre-s2 antigens. It was found that the positive rate for pre-s1 was up to 75%(15/20), being significantly higher than that of pre-s2 (35%) in the same group. More importantly the presence of pre..sl, rather than pre-s2, was in parallel with the presence of intrahepatic HBV DNA and HB_cAg. All findings suggest that pre-s1 was mainly expressed during HBV replication, indicating HBV replication. So detection of pre-s1 might substitute or complement HBe system and HBV DNA tests.