美国红栌的组织培养与快速繁殖技术研究

Tissue culture and rapid propagation of Continus coggygria

以美国红栌叶片为外植体材料,筛选各个培养阶段的最适培养基配方,结果表明:MS+6-BA 1.5 mg/L+NAA 0.5 mg/L对叶片诱导愈伤组织效果最好;MS+6-BA 1.0 mg/L为分化培养基的适宜配方;1/2 MS+IBA 1.0mg/L+蔗糖15 mg/L+琼脂5 g/L生根效果良好;采用平茬复壮技术可以达到越冬的良好效果.

The leaves of Continus coggygria were taken as explant,and the optimum media for each culture stage were selected out. The results showed that the medium MS＋6-BA 1.5 mg/L＋NAA 0.5 mg/L was the best for inducing callus from leaf ; medium MS＋6-BA 1.0 mg/L was the most suitable for propagation;medium 1/2 MS＋IBA 1.0 mg/L ＋surcrose 15g/L＋agar 5g/L was the optimum for rooting.