黄牛对抑制素pCISI基因免疫的反应性

Response of Cattle to Immunization with Inhibin pCISI Gene

为分析抑制素基因免疫对黄牛的影响,优化免疫方法,将3.0 mg抑制素真核融合表达质粒pCISI以不同纯度并配以不同的免疫佐剂分2次导入72头母黄牛体内,采用ELISA法检测血样中抗抑制素抗体效价。结果表明:抗体阳性牛占59.72%(43/72);添加佐剂的重组质粒较空质粒组免疫效果明显(P<0.05),普鲁卡因佐剂显著优于脂质体(P<0.05);高纯度疫苗组阳性牛的比例略高于低纯度组,但差异不明显(P>0.05)。这说明应用抑制素质粒pCISI免疫黄牛后可产生抗抑制素抗体。

To study the immunoreactive character and to improve the method of inhibin gene immunization in cattle, 3.0mg of inhibin fusion expressing plasmid pCISI with different purity and adjuvants was used to immunize the female cattle twice. Besides, the method of ELISA was adopted to examine the antibody. The results were as follows： 59.7% （43/72）of cattle were detected in positive antibody against inhibin; The effect of recombined plasmid with adjuvant was better than the one without adjuvant obviously（ P 〈 0.05）. The adjuvant procaine worked better than liposome evidently（ P 〈 0.05）; As to the rate of posi- tive antibody, group with highly purified inhibin pCISI was higher than that with commonly purified vaccine. However, the difference was not obvious（ P 〉 0.05）. These results suggested that inhibin plasmid pCISI could stimulate cattle to produce antibody against inhibin after gene immunization.