HPLC法测定盐酸米诺环素中甲醛的残留量

Determination of the Residual Formaldehyde in Minocycline Hydrochloridev by HPLC

目的 用高效液相色谱法测定盐酸米诺环素中甲醛的残留量，方法 采用C18色谱柱。柱温为30℃，以0．06mol·L^-1。磷酸溶液（pH2．85）-乙腈（50：50）为流动相，检测波长为336nm。结果 甲醛在0．21～2．47μg·mL^-1范围内线性关系良好。结论 本法准确、简便、快捷，适用于测定盐酸米诺环素中甲醛的残留量。

OBJECTIVE To determinate the residual Formaldehyde in Minocycline Hydrochloridev by HPLC. METHODS A Cls （4. 6mm × 250mm, 5μm）column was used with mobile phase of 0.06mol·L^-1 Phosphoric solution（pH2.85）-acetoni trile（50 ： 50） at detection wavelength of 336nm;The temperature of column was 30℃. RESULTS The calibration curve wad liner in the concentration rang of 0.21 ～ 2.47μg·mL^-1. CONCLUSION The HPLC method is accurate simple and rapid it can be used to determination the residual Formaldehyde in Minocycline Hydrochloridev.