高效液相色谱法测定呋塞米片的含量

Determination of Furosemide Tablets by HPLC

目的 建立高效液相色谱法测定呋塞米片含量的方法。方法 采用C18柱（250mm×4．6mm,5μm）色谱柱．以甲醇-水-磷酸盐缓冲液（500：493：7）为流动相，流速1.0ml·min^-1，检测波长为286nm。结果 呋塞米在0．597～3．980μg范围内进样量与峰面积呈丸好的线性关系（r=0.9998）;平均回收率为99．31%，RSD为1．01％，n=9。结论 本方法结果准确，重现性较好。

OBJECTIVE To establish a method for determination content of furosemide tables by HPLC. METHODS C18 column was used and methanol-water-phosphate buffer（500 ： 493 ： 7）was as mobil phase the flow rate was 1.0ml·min^-1, the detecting wavelength was at 286nm. RESULTS There was a good linear relationship between the concentration of furosemide and peak area within a range of 0.597～3. 980μg, r = 0. 9998. The average recover was 99.31% with RSD= 1.01% （n =9）. CONCLUSION The method is highly repeatable and accurate.