决明子质量标准的研究

Study of Quality Standard of Semen Cassiae

目的 建立决明子质量标准。方法 采用高效液相法时决明子中主要成分大黄素进行定量分析．采用Nova-pak C18柱（250mm×4．6mm，10μm）色谱拄，流动相为乙腈-0．1％磷酸（80：20），流速为1．0ml·min^-1，检测波长为254nm。结果 大黄素在0．1～0．5μg范围内呈良好的线性关系，回归方程为Y=653．95×（r=0．9999）．平均回收率为97．11％，RSD为1．28％，重现性RSD为1．89％。结论 本法简便，快速，灵敏，准确，重现性好，可作为决明子的质量标准。

OBJECTIVE To establish the quality standard for Semen cassiae. METHODS Emodin was determined by HPLC. The Nova-pakC18 column （250mm × 4. 6mm, 10μm） was used. The mobile phase was consisted of Acetonitrile-0. 1%phosphoric acid（80 ： 20）. The flow rate was1. 0ml · min^-1, the UV detection wavelength was 254nm. RESULTS The calibration curve in good Linenearity over the range of 0. 1～0.5μg, and regression equation Y= 653.95X（r= 0. 9999）. The average recovery was 97.11%, RSD was 1.28%, RSD and the reproduction was 1.89 %. CONCLUSION The method simple, quick, sensitive, accurate, reproducible and be used to control the quality of Semen cassiae.