HPLC法测定血清中苯巴比妥和卡马西平的浓度

Simultaneous Determination of Phenobarbital and Carbamazepine in Serum by HPLC

目的 建立人血清中苯巴比妥、卡马西平浓度的高效液相色谱含量测定方法。方法 采用ODS-Hypersil（4．6mm×100mm，5μm）色谱柱，以甲醇：水（55：45）为流动相。紫外检测波长为254nm，以苯妥英为内标。结果 苯巴比妥在2．5～40μg·mL^-1浓度范围内线性关系良好（r=0.9996），平均回收率为100．69％，RSD为1．8％；卡马西平在1．25～20μg·mL^-1浓度范围内线性关系良好（r=0．9995），平均回收率为98．67％，RSD为2．1％。日内和日间RSD均不大于3％。结论 本方法准确、快速、简便，可作为苯巴比妥和卡马西平血药浓度监测的常规方法。

OBJECTIVE To establish an HPLC method for the determination of Phenobarbital and Carbamazpine in human plasma. METHODS The separation was pertormed on a ODS Hypersil column. The mobile phase was composed of methanol-water（55：45）. The detective wavelength was at 254nm. Phenytoin was used as the internal standard. RESULTS The calibration curves was linear in the range of 2. 5～40μg·mL^-1 for Phenobarbital （r=0.9996） and 1.25% 20 μg·mL^-1 for Carbamazepine （r = 0. 9995）,respectively. The average recovery of Phenobarbital was 100.69% and that of Carbamazpine was 98.67%. Intra-day RSD and inter-day RSD were less than 3 %. CONCLUSION The method is accurate,rapid,and convenient. It can be used for a routine method to determinate the plasma concentration of PB and CBZ.