细胞外信号调节激酶在油酸型急性肺损伤大鼠肺组织中的表达及意义

Expression condition of extracellular signal regulated protein kinase in rats with oleic acid-induced acute lung injury and its significance

目的观察油酸致伤后大鼠肺组织细胞外信号调节激酶(ERK)和磷酸化ERK(P-ERK)与肿瘤坏死因子(TNF-α)及白介素-6(IL-6)释放的关系。方法健康雄性SD大鼠60只,随机分为油酸1h组,油酸2h组,油酸4h组,油酸24h组和对照组,观察各组肺组织病理学改变,免疫组化法和蛋白定量法(Western blot)测定肺内ERK,P-ERK表达,放免法检测血清、BALF、肺组织匀浆液中TNF-α及IL-6水平。结果油酸组大鼠支气管、肺泡上皮细胞、肺血管内皮细胞、肺泡巨噬细胞上表达ERK和P-ERK明显增多,血清、BALF、肺组织匀浆液中TNF-α及IL-6水平明显升高,TNF-α与ERK和P-ERK表达峰值时间均为1h,IL-6表达峰值时间为4h。结论ERK信号通路参与调节油酸型急性肺损伤大鼠IL-6的释放过程,与TNF-α为互相促进的关系。

Objective To observe the ralationship of extracellular signal regulated protein kinase （ERK） and phosphorylating ERK with TNF - αtand IL - 6 in rats with acute lung injury induced by oleic acid. Methods 60 healthy male SD rats randomly divided into 5 groups： Oleic acid 1 h group, Oleic acid 2 h group, Oleic acid 4 h group, Oleic acid 24 h group and control group. Pathology changement of the lung was investigated. ERK and P - ERK expression condition was determined by Western blot and immunohistochemical method. Levels of TNF -α and IL -6 in the rat＇s serum, the lung tissue and BALF was examined by the Radioimmunnol method. Results ERK and P - ERK in bronchus epithelial cells, alveolar epithelial cells, pulmonary vessel endothelial cells and macrophage cell was highly expressed in the lung of OG rats ,The level of TNF -α and IL- 6 in serum, the lung tissue and BALF increased obviously after rat being injected oleic and the peek value time of ERK, P- ERK and TNF -α was same in 1 h after injured, the peek value time of IL - 6 was 4 h. Conclusions ERK signal transaction path participated IL - 6 releasion process while with TNF -α for mutual promotive relationship.