二巯丙磺钠对二甲基甲酰胺急性中毒小鼠SOD XOD CAT的影响

Influence of sodium dimercaptopropan on SOD,XOD and CAT in acute dimethylformamide (DMF) poisoning mice

目的观察二甲基甲酰胺(DMF)中毒对小鼠氧化酶、抗氧化酶的影响及二巯丙磺钠(Na-DMPS)的保护和治疗作用。方法①48只小鼠,予3g/kg DMF灌胃染毒(ig)后6、12、24、48、72h摘眼球取血及取肝左叶,连同正常组测血及肝匀浆中XOD、SOD、CAT活力。②64只小鼠,分为正常组、中毒对照组、Na-DMPS保护治疗组和单纯保护治疗组,分二批于ig后6h摘眼球取血,ig后24h取肝左叶,测血浆超氧化物歧化酶(SOD)及肝匀浆中SOD、黄嘌呤氧化酶(XOD)、过氧化氢酶(CAT)活力。结果与正常组比较,3g/kg DMF灌胃后血SOD于6h降低(P<0.01),血XOD于72h升高(P<0.05),肝匀浆XOD、SOD、CAT于24h升高(P<0.01)。Na-DMPS保护治疗后,肝组织SOD、XOD较中毒对照组明显下降,差异有统计学意义(P<0.01)。结论Na-DMPS可降低肝组织XOD活性,并对肝脏的SOD升高有降低作用,恢复体内氧化/抗氧化系统的平衡,具有保护肝功能作用,能用于DMF急性中毒的治疗。

Objective To observe the influence of oxidase and antioxidase in acute dimethylformamide （DMF） poisoning mice and treatment effect of sodium dimercaptopropan （Na- DMPS）. Methods ① Blood and left liver were taken from 48 mice,which were divided into 6 groups and were intragastric（ig） with the DMF dasage of 3 g/kg,at the time 0,6,12,24,48,72 h after ig. The activities of XOD,SOD, CAT were tested. ②64 mice ig with the method mentioned above were divided into 4 groups,then blood and left liver were taken at the time of 6,24 h after ig in each of 32 mice. The activities of XOD,SOD,CAT were detected. Results Compared with normal group,SOD in blood decreased at 6 h after ig （P 〈 0.01 ）,XOD in blood increased at 72 h （ P 〈0. 05 ）, XOD, SOD, CAT of liver increased at 24 h （ P 〈 0. 01 ）, and returned to normal in 48-72 h. There were very significantly difference between poisoning group and Na- DMPS treated group （P 〈0.01 ）. Conclusions XOD increased in blood and liver,SOD and CAT increased in liver as well,while SOD decreased in blood in Acute DMF poisoning mice. SOD and XOD could decrease by the treatment of Na - DMPS,the balance of oxidase and antioxidase was renewed and the liver was protected as well. It was feasible that Na - DMPS was used to the treatment of acute DMF poisoning.