凋亡抑制蛋白Survivin、bcl-2在非小细胞肺癌中的表达及意义

Expression of inhibitor of apoptosis protein Survivin and bcl-2 in non-small-cell lung cancer and its significance)

目的:探讨凋亡抑制蛋白Survivin、bcl-2蛋白在非小细胞肺癌(NSCLC)组织中的表达与不同临床病理特征的关系及二者在肺癌发生过程中可能存在的相关性。方法:采用免疫组化法的链霉菌抗生物素过氧化酶(S-P)法检测80例NSCLC组织和20例正常肺组织中Survivin、bcl-2蛋白表达情况。结果:非小细胞肺癌组织中Survivin、bcl-2蛋白阳性表达率分别为61.3%、50%,而在肺良性病变组织中阳性表达率分别为0%、10%,两者在两组中的表达均有显著性差异(P<0.05);Survivin蛋白在～期肺癌组织中阳性表达率72.0%显著高于I～II期肺癌组织的43.3%(P<0.05),bcl-2蛋白在鳞癌组的阳性表达率62.2%高于腺癌组的34.3%(P<0.05);Survivin、bcl-2蛋白在肺癌组织中的表达呈正相关关系。结论:Survivin蛋白表达与肺癌的TNM分期密切相关,Survivin、bcl-2可以作为判断病情和评价预后的指标。

Objective, To study the relationship between the expression of inhibitor of apoptosis protein Survivin and bcl-2 in NSCLC and its clinicopathological characteristic and to discuss their potential relevance existing in lung carcinoma progression. Methods： The expression of Survivin and bcl-2 protein was detected in 80 cases of NSCLC tissue and 20 cases of normal lung tissue by immunohistochemical streptavidin-peroxidase （SP） method. Results： The positive rates of Survivin and bcl-2 in lung carcinoma were 61.3% and 50 % respectively. The positive rates of Survivin and bcl-2 in normal lung tissues were 0% and 10% respectively. There were significant differences in the expression of Survivin and bcl-2 between lung carcinoma group and normal lung tissues group（P〈0.05）. The positive rate of Survivin in stage Ⅱ-Ⅳ lung carcinoma （72.0%） was significantly higher than that of Survivin in stage Ⅰ-Ⅱ lung carcinoma（43.3%）（P〈0. 05）. The positive rate of bcl-2 in squamous carcinoma（62. 2%） was significantly higher than that of adenocarcinoma （34. 3%）（P〈0. 05）. There was positive correlation existing in the expression of Survivin and bcl-2 in lung carcinoma. Conclusion：The expression of Survivin and bcl-2 may play a role in the pathway of carcinogenesis and be identified as a definite diagnostic and evaluating prognostic marker in NSCLC.