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巴氏杆菌可溶性抗原的提取及蛋白浓度测定 被引量：1

Extract of Soluble Antigens and Protein Contents Measurement in Pasteurell
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摘要 改良EDTA法提取巴氏杆菌内可溶性抗原成分,经透析浓缩后保存,并取用考马斯亮蓝法测定其浓度.结果得回归方程为y=0.5589x-0.0262,r=0.9990.由吸光度推出样品蛋白浓度为1.643mg/mL,而蛋白检测仪为1.412mg/mL. The soluble antigens of Pasteurella were extracted by improved EDTA method and were preserved after having been dialysed and concentrated. The consistence of the soluble antigens were measured by Coomassie Brilliant Blue method. Then the tropic equation came out to be： y= 0. 5589x- 0. 0262, r= 0. 9990. So the protein consistency of tile sample was figured out to be 1. 643 mg/mL according to the absorbence, While the result come from Pritein Measureing Instrument was 1. 412 mg/mL.
作者 毛东风 刘翊中 佟辉 孙会芳 冯若飞 王建
机构地区 西北民族大学生命科学与工程学院
出处 《西北民族大学学报（自然科学版）》 2006年第1期64-66,共3页 Journal of Northwest Minzu University(Natural Science)
关键词 EDTA 考马斯亮蓝 ELISA EDTA Coomassie Brilliant Blue ELISA
分类号 S852.6 [农业科学—基础兽医学]
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参考文献2

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共引文献10

同被引文献13

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西北民族大学学报（自然科学版）
2006年 第1期

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