用微卫星标记辅助鉴定几种小麦缺体—四体

Simple Sequence Repeats-Assisted Identification of Several Nulli-Tetrasomes in Wheat

用已准确定位到小麦染色体上的部分SSR标记辅助鉴定3个中国春缺体-四体材料:N2AT2B、N2BT2D、N2DT2A。以中国春小麦为对照,首先选用缺失染色体上的SSR引物,PCR扩增结果表明3个材料均缺失该对染色体;进一步用分布于小麦21条染色体上的SSR引物进行PCR扩增,发现除缺失染色体外的其余染色体均有扩增,说明该材料至少有其余20对染色体中的每一条;最后用缺体-四体材料对24个新开发未知位点的EST-SSRs标记进行染色体定位,结果其中10个EST-SSRs位点被分别定位在小麦2A、2B、2D染色体上。

SSRs（simple sequence repeats）, or microsatellites, which were already assigned to wheat chromsomes, were used to test the authenticity of 3 nulli-tetrosomes lines of wheat： N2AT2B, N2BT2D,N2DT2A. Firstly, PCR amplified products implied that they were nullisomes. Secondly, after evaluating the other chromosomes except the lost chromosomes ,they were truly nulli-tetrosome lines. And the identified lines were then used to localize some newly designed and unkown EST -SSRs loci on the chromosomes. A total of 10 microsatellites loci were derived from the 10 primer pairs and separately located onto the wheat chromosomes： 2A,2B,2D. And this procedure proved to be convenient to handle for identification of Nulli-Tetrasomes in wheat.