TDZ和6-BA对枣树继代培养的影响

Effect of TDZ and 6-BA on Subculture of Zizyphus jujuba Mill

以狗头枣和骏枣试管苗为材料，研究了TDZ和6-BA在枣树试管苗继代培养中的效应并筛选了适宜组合。结果表明，在枣试管苗继代培养中，TDZ与6-BA分别与IBA配合使用时，附加6-BA 1．2～1．6mg／L比较适合；TDZ的活性高于6-BA。其诱导的不定芽比6-BA的多但芽曲质量显著降低。二者结合使用则可显著促进不定芽的增殖并提高成苗质量，在CY＋TDZ 0．005～0．01mg／L＋6-BA1．0mg／L＋IBA0．2mg／L的组合培养基上可获得最佳繁殖效果。TDZ与IBA配合使用时能抑制试管枣苗生根，但可促使茎段愈伤组织的诱导。说明TDZ具有强烈地刺激细胞分裂的作用，并且在枣试管苗继代培养中与6-BA具有很好的协同促进作用。

Use test-tube seedlings of Chinese date Goutouzao and Junzao as explants, effect of TDZ and 6-BA on subculture of Chinese date were studied and selected its suitable composition in subculture. The results showed that in subculture added 1. 241.6 mg/L 6- BA was suitable, then them respectively together with IBA. Activity of TDZ was higher, and it induced bud, more bud, but quality of seedlings cut down, as compare with 6- BA. TDZ and 6-BA blended with IBA can remarkable pro mote propagate of buds and quality of grow into seedlings; The best effect obtained on the medium containing CY ＋ 0. 005-0.01 mg/L TDZ ＋ 1.0 mg/L 6- BA ＋ 0.2 mg/L IBA. Then TDZ blended with IBA can check growth of root of test-tube seedlings but can promote induction of ealli in stem segment. It showed that the TDZ have effects of strongly stimulated cell division and with 6-BA have best cooperation effects.