平喘灵冲剂对小鼠耳廓微循环的影响

Effect of Pingchuanlingchongji on the Auricular Microcirculation in Mice

目的观察平喘灵冲剂对小鼠耳廓微循环的影响并论证平喘灵冲剂的活血化瘀作用。方法将50只小鼠随机分为5组,其中3组分别给予平喘灵冲剂小(1.1g/kg)、大剂量(2.2g/kg)及阿司匹林(0.25g/kg)灌胃,其余2组给予生理盐水灌胃,连续7d。于末次给药后1h,除正常组外,每鼠尾静脉注射垂体后叶注射液,然后观察小鼠耳廓微循环微动脉口径、微静脉口径、血流速度的变化。结果平喘灵冲剂能显著扩大小鼠耳廓微循环微动脉口径及微静脉口径、能提高小鼠耳廓微循环血流速度,与对照组比较均有显著性差异(P<001)。结论平喘灵冲剂对小鼠耳廓微循环有明显的改善作用。

Objective To observe the effect of Pingchuanlingchongji （PCL） on the auricular microcirculation of mice and to investigate the effect mechanism of the treatment for asthma. Methods 50 mice were randomly divided into five groups, group 1 and group 2 were given 0.9% NS （25 mL/kg） as control group; group 3 was given aspirin （0.25 g/kg）, 4 group was given low-dose （1.1 g/kg） PCL, group 5 was given high-dose （2.2 g/kg） PCL. Last for seven days. We injected Posterior Pituitary Injection （0.5 U/mL） on the rear vein of 2-5 group mices after the last time of given druy. The diameter of micro-artery in mice auricular, the diameter of micro-vein and blood flow speed were observed. Results As compared with contral group, PCL could expand significantly the diameter of micro-artery and the diameter of micro-vein in mice auricular （P〈0.01）. PCL could improve significantly the blood flow speed in mice auricular （P〈0.01）. Conclusion PCL can obviously improve the auricular microcirculation of mice.