中药对解脲支原体临床分离株的体外抑制试验研究

Study on metabolix inhibition test in vitro to the clinical strains of ureaplasma urealyticum

目的 研究6种单种中草药及二种复方中草药对解脲支原体（Uu）临床分离株的作用并确定它们的最低抑菌浓度（MIC）。方法 临床采集标本后体外培养获得Uu临床分离株，应用微量稀释法测定中草药对解脲支原体临床株的单独作用及部分联合作用，并测定了其最低抑菌浓度（MIC）。结果 6种中草药对解脲支原体临床分离株的最低抑菌浓度MIC范围（生药）分别为：五倍子：0．313—1．25g／L，诃子：1．25—5．00g／L，黄柏：1．25—5．00g／L，栀子：0．625—5．00g／L，赤芍：1．25-2．50g／L，板兰根：10．00—40．00g／L，五倍子＋诃子：1．25—5．00g／L。五倍子＋赤芍：1．25—5．00g／L。复方中草药八正散对解脲脲原体临床分离株的MIC范围（生药）为：2．50—20．00g/L，五淋散为：2．50—20．00g/L。结论 五倍子，黄柏，诃子，栀子，赤芍等具有较强的抑制解脲脲原体临床分离株的作用，传统中药八正散及五淋散也有一定的抑制解脲脲原体临床分离株的作用。应进一步研究中药抗Uu的作用及其机制。

Aim To explore the antibacterial effect of Chinese crude （ including 6 single drugs and 2 mixed drugs） to the clinical strains of ureaplasma urealyticu and determine their minimal inhibitory concentration. Methods Collect the specieses from clinical patients and culture. To test the different effects on the clinical strains of ureaplasma urealyticu, metabolix inhibition test were applied to the assays. At the same time , minimal inhibitory concentration of the drugs to the clinical strains of ureaplasma urealyticu were assayed. Results Six drugs＇ minimal inhibitory concentration to the clinical strains of ureaplasma urealyticu, Chinese gall： 0.313 - 1.25 g/L , chebulae： 0.25 - 5. 00 g/L, cortex phellodendril. 25 - 5. 00 g/L gardenia： 0. 625 - 5.00 g/L, paeoniae radix： 1.25 - 2.50 g/L, banlangeng 10. 00 - 40.00 g/L. Chinese gall ＋ chebulae ： 1.25 - 5.00 g/L, Chinese gall ＋ paeoniae radix ： 1.25 - 5.00 g/L. The mixed drugs＇metabolix inhibition test ： bazhengsan 2.50 - 20.00 g/L,wulinsan2.50 - 20.00 g/L. Conclusions Chinese gall, chebulae, cortex phellodendril gardenia, paeoniae radix, et al exert the stronger antibacteria effect on the clinical strains of ureaplasma urealyticu. Traditional Chinese medicine,bazhengsan and wulinsan have the antibacteria effect to some extent, h is worthwhile to study further on the effects and mechanism of chinese crude drugs＇antibacteria to ureaplasma urealyticum.