肾疏宁对肾小球硬化大鼠肝细胞生长因子的影响

Effects of Shenshuning Recipe on expression of hepatocyte growth factor in rats with glomerulosclerosis

目的:探讨肾疏宁对肾小球硬化大鼠肝细胞生长因子(hepatocyte growth factor,HGF)的影响。方法:采用单侧肾切除及尾静脉注射阿霉素方法建立大鼠肾小球硬化模型。42只造模大鼠随机分为模型组,肾疏宁治疗组和苯那普利治疗组,另设8只大鼠作为假手术组。肾疏宁治疗组和苯那普利治疗组分别予以肾疏宁和苯那普利治疗8周,其余两组予以等量蒸馏水灌胃。治疗8周末,测定大鼠24 h尿蛋白定量(urine protein,Upr)、血清肌酐(creatinine,Cr)和尿素氮(blood urea nitrogen,BUN);光镜下观察各组大鼠肾组织病理形态学改变,比较各组大鼠肾小球毛细血管直径、系膜基质和肾小球硬化指数;采用逆转录-聚合酶链反应(reverse transcription-polymerase chain reaction,RT-PCR)技术检测肾组织HGF mRNA的表达水平。结果:模型组大鼠24 h Upr、血清Cr和BUN较假手术组明显升高(P<0.01);肾小球硬化呈弥漫性分布,肾小球内部分毛细血管腔缩小或塌陷闭塞,系膜基质增多,系膜区增宽,肾小球硬化指数3.32±0.35;肾组织HGF mRNA的表达明显下调(P<0.05)。肾疏宁治疗组和苯那普利治疗组大鼠24 h Upr、血清Cr和BUN较模型组明显降低(P<0.01);系膜细胞增生和系膜基质明显减少,毛细血管开放好转,肾小球硬化指数降低(P<0.01);肾组织HGF mRNA的表达明显上调(P<0.01)。结论:肾疏宁可以上调肾组织HGF mRNA的表达,减少系膜基质,改善肾脏病理形态,延缓肾小球硬化的进程。

Objective： To investigate the effects of Shenshuning Recipe （SSNR） on gene expression of hepatocyte growth factor （HGF） in renal tissues in rats with glomerulosclerosis. Methods： Glomerulosclerosis was induced in 42 rats by unilateral nephrectomy and intravenous injection of doxorubicin. Then these 42 rats were randomly divided into three groups： untreated group, SSNR-treated group and benazepril-treated group. Another eight rats were included into sham-operation group. The rats in the SSNR-treated group and the benazepril-treated group were fed SSNR or benazepril respectively for 8 weeks, The levels of 24 h urine protein （Upr）, serum creatinine （Cr） and blood urea nitrogen （BUN） of rats in each group were examined. The renal morphological changes were observed under microscope, and the diameter of glomerular capillary, mesangial matrix and glomerulosclerosis index were analyzed by image analysis software. Reverse transcription-polymerase chain reaction （RT-PCR） method was tissues. Results： The levels of 24 h Upr, serum Or and used to detect the gene expression of HGF in the renal BUN in the untreated group were remarkably increased than those in the sham-operation group （P〈0.01）. The pathological morphological changes in the untreated group showed that the glomerulosclerosis was diffused around the renal tissue and the capillaries were shrunk. The expression level of mesangial matrix was up-regulated and the glomerulosclerosis index was 3.32 ±0.35. The expression level of HGF mRNA in the untreated group was obviously lower than that in the sham-operation group （P〈0.05）. The levels of 24 h Upr, serum Cr and BUN in the SSNR-treated group and the benazepriltreated group were remarkably decreased as compared with those in the untreated group, while the expression levels of HGF mRNA were both obviously higher than that in the untreated group （P〈0.01）. The pathological morphological changes in the SSNR-treated group and the benazepril-treated group were both alleviated. There was no significant difference in therapeutic effect between the SSNR-treated group and the benazepril-treated group. Conclusion： Shenshuning Recipe can up-regulate the expression of HGF mRNA, decrease the mesangial matrix, and improve the renal function, so that it may retard the development of glomerulosclerosis.