摘要
Hyaluronan may be used in tissue engineering as a scaffold, and since the scaffold should degrade after the cells have proliferated, and heat treatment is a method to achieve low molecular weight material. In this essay, we developed a new method to choose the characteristic heat treatment temperature, and applied a simple method to prepare the hyaluronan scaffold. We have acquired the TG and DTA curves of hyaluronan by thermal analysis, according to which we selected 310 ℃ ,375 ℃ and 500 ℃ as the heat treatment points. After heat treatment, test the infrared spectrum of the powder respectively. In conclusion, the scaffold forrued by lyophilization exhibits a porous structure, and the occurrence of new groups after heating assumes the change of the molecular chain.
Hyaluronan may be used in tissue engineering as a scaffold, and since the scaffold should degrade after the cells have proliferated, and heat treatment is a method to achieve low molecular weight material. In this essay, we developed a new method to choose the characteristic heat treatment temperature, and applied a simple method to prepare the hyaluronan scaffold. We have acquired the TG and DTA curves of hyaluronan by thermal analysis, according to which we selected 310 ℃ ,375 ℃ and 500 ℃ as the heat treatment points. After heat treatment, test the infrared spectrum of the powder respectively. In conclusion, the scaffold forrued by lyophilization exhibits a porous structure, and the occurrence of new groups after heating assumes the change of the molecular chain.
基金
Fundedby863Projectof"theTenthFiveyearPlan"(No.2002AA205091)
