摘要
In order to provide valuable information for the design of new calcium phosphate bone repair materials,bone tissue engineering scaffold materials, and other dinical application, the interaction between calcium phosphate materials and proteins were investigated. The adsorption of the calcium phosphate ceramic to the protein was investigated by using FT-IR, XPS, SEM, and SDS-PAGE. As the results shown, the proteins were strongly adsorbed adsorbed the CPC, and a shift of the feature peak of the protein and also a chemical shift in the Ca2p and O1s bind energy of CPC was observed. This indicated that the acidic amino-group and alkaline amino- residue on the proteins＇ surface bonded to the Ca^2＋ in the β- TCP crystal by ionic bond and the proteins＇ alkaline amino groups to the oxygen in PO4^3＋ by hydrogen bond and electrostatic attraction. The adsorption mechanism of the protein in the CPC can be described as three adsorption layers ： irreversible chemical adsorption layer, physical adsorption layer and biomineralized adsorption layer.
In order to provide valuable information for the design of new calcium phosphate bone repair materials,bone tissue engineering scaffold materials, and other dinical application, the interaction between calcium phosphate materials and proteins were investigated. The adsorption of the calcium phosphate ceramic to the protein was investigated by using FT-IR, XPS, SEM, and SDS-PAGE. As the results shown, the proteins were strongly adsorbed adsorbed the CPC, and a shift of the feature peak of the protein and also a chemical shift in the Ca2p and O1s bind energy of CPC was observed. This indicated that the acidic amino-group and alkaline amino- residue on the proteins＇ surface bonded to the Ca^2＋ in the β- TCP crystal by ionic bond and the proteins＇ alkaline amino groups to the oxygen in PO4^3＋ by hydrogen bond and electrostatic attraction. The adsorption mechanism of the protein in the CPC can be described as three adsorption layers ： irreversible chemical adsorption layer, physical adsorption layer and biomineralized adsorption layer.
基金
SurportedbyKeyTechnologiesR&DProgramofHubeiProvince(2004AA303B03)
