广东省实施野生动物市场干预后SARS冠状病毒污染情况分析

SARS-CoV contamination in wild animal market after intervention in Guangdong province

目的了解广东省实施野生动物市场干预后野生动物市场SARS冠状病毒污染情况,为防控SARS疫情提供科学依据。方法于2004年9月至2005年5月,每月在广州市两家经营野生动物的场所分别采集动物市场外环境样本和各种动物样本(咽拭子、肛拭子、粪便等),用Realtime-PCR法检测SARS-CoV核酸基因片段。结果2004年1月起,广东省对动物市场进行干预,在全省范围内关闭野生动物交易市场,全面禁止果子狸等野生动物的饲养、运输、销售、屠宰和食用。市场干预后,检测销售飞禽类、水禽类、畜类、兽类、水产类等的动物市场外环境样本共417份,检测动物市场蛇、水产、黄犭京、竹鼠、兔、猫、鸡、鸭、鸽子等动物样本共1 160份,均未检出SARS-CoV核酸片断。结论实施对野生动物市场干预,能有效降低动物市场各种动物SARS-CoV的携带率,对预防SARS的发生和传播起到了重要作用。

Objective To investigate SARS-CoV contamination in animals and environment in wild animal markets after a series of intervention. Methods Samples from animal and environment were collected from Sep. 2004 to May. 2005 and Real-time reverse transcription polymerase chain reaction （RT-PCR） was used to detect SARS-CoV gene. Results All of 417 environment and 1 160 animal samples were negative. Condusion The intervention measures in animal market management were effective in elimination of SARS-CoV contamination.