Antiviral effect of Chinese medicine jiaweisinisan in hepatitis B virus transgenic mice

AIM： To study the antiviral effect of Chinese medicine jiaweisinisan （JWSNS） on hepatitis B virus （HBV） infection in transgenic mice （TGM）. METHODS： Twenty two 6-8 wk old HBV TGM in the third generation were divided into TGM control group and TGM treated group randomly. The normal control group included ten normal BC 57L/6 mice at the same age. The mice in treated group were administrated with JWSNS at the concentration of 4 g/mL and the dosage of 50 g/kg per d for 30 d, while the mice in TGM control group and normal control group were administrated with normal saline at the same dosage and the same time. Polymerase chain reaction （PCR） was used to assess the contents of HBV DNA in serum of HBV TGM before and after treatments, whereas blot hybridization was utilized to measure the contents of HBV DNA in the liver of both HBV TGM and normal BC 57L/6 mice. RESULTS： The levels of serum HBV DNA in TGM treated group were remarkably decreased after the treatment of JWSNS （7.662±0.78 vs 5.22±3.14, P〈0.05）, while there was no obvious change after administration of normal saline in TGM control group （7.125±4.26 vs 8.932 ± 5.12, P〉 0.05）. The OD values of HBV DNA in the livers of the mice in TGM treated group were significantly lower than those of TGM control group （0.274±0.096 vs 0.432 ± 0.119, P 〈 0.01）. CONCLUSION： JWSNS exerts suppressive effects on HBV DNA in the serum and liver of TGM.

瞄准：在转基因的老鼠(TGM ) 在肝炎 B (HBV ) 感染上学习中国药 jiaweisinisan (JWSNS ) 的抗病毒的效果。方法：22 6-8 wk 在第三代的旧 HBV TGM 被划分成控制组织的 TGM， TGM 随机对待组。正常控制组在一样的年龄包括了十只正常 BC 57L/6 老鼠。在对待的组的鼠标为 30 d 每 d 在 4 g/mL 的集中和 50 g/kg 的剂量与 JWSNS 被管理，当在 TGM 控制的鼠标组织，正常控制组在一样的剂量和一样的时间与生理盐水被管理时。聚合酶链反应(PCR ) 被用来在处理前后在 HBV TGM 的浆液估计 HBV DNA 的内容，而污点杂交被利用在 HBV TGM 和正常 BC 57L/6 老鼠的肝测量 HBV DNA 的内容。结果：在对待的 TGM 的 DNA 组织的浆液 HBV 的层次显著地在 JWSNS 的处理以后被减少(7.662+/-0.78 对 5.22+/-3.14， P < 0.05 ) 当在在 TGM 的生理盐水的管理以后没有明显的变化时，控制组织(7.125+/-4.26 对 8.932+/-5.12， P > 0.05 ) 。在在对待的 TGM 的鼠标的肝的 DNA 组织的 HBV 的 OD 值是比 TGM 控制组的那些显著地低的(0.274+/-0.096 对 0.432+/-0.119， P < 0.01 ) 。结论：JWSNS 在 TGM 的浆液和肝在 HBV DNA 上施加镇压效果。