宫颈癌单克隆抗体AU_(14-1)介导效应细胞对靶细胞的细胞毒

Cytotoxicity of effector cells targeting tumor cells mediated by monoclonal antibody AU_(14-1) against experimental uterine cervical carcinoma

目的:研究单克隆抗体AU14-1介导的细胞毒作用对小鼠宫颈癌(U14)细胞的杀伤效果。方法:以小鼠淋巴因子激活的杀伤细胞(LAK)、脾淋巴细胞及巨噬细胞为效应细胞,应用MTT比色法测试在单克隆抗体AU14-1介导下对小鼠宫颈癌(U14)细胞的体外细胞毒作用。结果:3种效应细胞对于经单克隆抗体AU14-1处理的肿瘤细胞的杀伤率显著高于未经AU14-1处理的肿瘤细胞(P<0.01);以LAK的ADCC作用最强,巨噬细胞次之,脾淋巴细胞最弱(P<0.01)。结论:单克隆抗体AU14-1能通过抗体依赖细胞介导细胞毒(ADCC)发挥淋巴样细胞、巨噬细胞及LAK细胞对靶肿瘤细胞的杀伤作用;提示特异性宫颈癌单克隆抗体AU14-1介导的ADCC在治疗宫颈癌中可能是一种有潜在价值的方法。

AIM： To investigate the effect of monoclonal antibody （McAb） AU14-1 inediated cytotoxicity against cervical cancer U14 cell in vitro, METHODS： MTr colorimetric assay was applied to study the McAb AU14-1 mediated cytotoxicity of effector cells including splenocytes, peritoneal macrophages and LAK to U14 cells. RESULTS： The eytotoxieity mediated by each effector cells to the U14 cells treated with McAb AU14-1 was significantly higher than those not treated with it （ P 〈 0.01 ） and the ADCC activity of the LAK was the strongest among them, the macrophagas was the second and the splenocytes the weakest （ P 〈 0.01）. CONCLUSION： The study indicates that McAb AU14-1 against cervical carcinoma may mediate the ADCC of the splenocytes, the peritoneal macrophages and the LAK to U14 cells, its ability to direct ADCC might suggestes this antibody may be a promising candidate for potential cervical cancer therapy.