蝉拟青霉总多糖对免疫抑制大鼠组织器官免疫功能调节的实验研究

Paecilomyces cicadidae total polysaccharides regulates immune function in immunosuppressed rats

目的:观察蝉拟青霉总多糖对免疫抑制大鼠组织器官免疫功能调节和自由基代谢的影响,探讨其对免疫抑制大鼠组织器官的免疫调节和抗衰老作用。方法:以速率法在全自动生化分析仪上测定乳酸脱氢酶(LDH)活力;以鸟氨酸的生成判断精氨酸酶(arginase)的活力;以硫代巴比妥酸-醋酸法测定脂质过氧化物(LPO)含量;以Ell-man试剂测定还原型谷胱甘肽(GSH)水平。结果:蝉拟青霉总多糖使环磷酰胺所致免疫抑制大鼠组织细胞内LDH(肾、胸腺)、arginase(肝、脾、胸腺)活力升高,具有显著差异(均P<0.01);使LPO(肾、脾、胸腺)的含量下降(P<0.05或P<0.01);使环磷酰胺所致的肝、脾内低下的还原型谷胱甘肽(GSH)升高(均P<0.01)。结论:蝉拟青霉总多糖能增强免疫抑制大鼠组织器官的免疫功能和加速自由基的代谢。

AIM： To study the effects of paecilomyces cicadidae total polysaccharides on the immune function and free radical scavenging of tissues in immunosuppressed rats. METHODS： The activities of lactate dehydrngenase （LDH） and arginase （Arg） in liver, kidney, spleen, thymus of immunosuppressed rats were detected by automatic biochemistry analyzer. The content of lipid peroxide （LPO） and the reduced glutathione （GSH） level in hver, kidney, spleen, thymus of immunosuppressed rats were observed. RESULTS： The activity of the LDH, Arg and GSH level were significantly increased, and the LPO content were significantly declined in liver, kidney, spleen, thymus of immunosuppressed rats induced by cyclophosphamide. CONCLUSION： Paecilomyces cicadidae total pelysaccharides promote immune function and the ability of free radical scavenging of tissues in immunosuppressed rats.