双歧杆菌对大鼠腹腔巨噬细胞NF-κB和IκB的影响

Influence of bifidobacterium on NF-κB and IκB in murine peritoneal macrophages

目的:探讨双歧杆菌对大鼠腹腔巨噬细胞核转录因子NF-κB和IκB调控。方法:收集大鼠腹腔巨噬细胞,实验分为正常对照组、双歧杆菌刺激组,以不同浓度的双歧杆菌106、1071、08和109cells/L刺激细胞60 min,或以108cells/L的浓度刺激细胞不同时间(15、30、601、20和180 min),分别收集细胞以提取总蛋白及核蛋白。用凝胶电泳迁移分析法(EMSA)测定NF-κB的结合活性;用Western印迹分析巨噬细胞胞浆内相应的IκBα蛋白表达。结果:双歧杆菌处理后,巨噬细胞NF-κB向核内转移,结合活性明显增加,相应的IκBα表达降低。结论:双歧杆菌可以通过激活腹腔巨噬细胞核转录因子NF-κB来发挥调作控用。

AIM： To explore the regulatory effect of bifitdobacteria adolescence on NF-κB in murine peritoneal maerophages （PMs）. METHODS： Maerophages were collected and were divided into tow groups, normal control group and bifidobacterium stimulation groups. The cells were fixed at 60 min after stimulated with bifidobacterium at 10^6, 10^7, 10^8 and 10^9 cells/ L, or fixed respectively at 15, 30, 60, 120 and 180 min after stimulated with bifidobacterium at 10^8 cells/L, then the total protein and nueleoprotein were extracted. The activities of NF-κB and IκBα were measured with the methods of MSA and Western blotting. RESULTS： NF-κB activity markedly increased and reached the peak at 0.5 h after stimulation, while IκBα markedly decreased at same time. CONCLUSION： NF-κB activity is markedly activated by bifidobacterium. NF-κB pathway participates in the regulation of peritoneal macrophage in this process.