肾上腺髓质素2对大鼠脑微血管内皮细胞增殖的影响

Effects of adrenomedullin 2 on proliferation of microvascular endothelial cells from the rat cerebral cortex

目的:研究肾上腺髓质素2(AM2)对大鼠脑微血管内皮细胞增殖的影响。方法:分离并培养SD大鼠脑微血管内皮细胞,经Ⅷ因子相关抗原的抗体鉴定和常规处理后,随机分为对照、AM2(10-7mol/L1、0-8mol/L和10-9mol/L)、ADM、ADM+AM2、10%胎牛血清和10%胎牛血清+AM210-7mol/L等8组,以[3H]-TdR掺入法测定MEC增殖反应。结果:10-7-10-9mol/L各浓度AM2、ADM以及AM2和ADM合用对于静止状态的脑微血管内皮细胞[3H]-TdR掺入与对照组比较均无明显差异(均P>0.05)。10%胎牛血清刺激组脑微血管内皮细胞[3H]-TdR掺入比对照组高87.5%(P<0.05),10-7mol/L AM2抑制胎牛血清诱导的脑微血管内皮细胞[3H]-TdR掺入增加(P<0.05)。结论:AM2抑制血清诱导的脑微血管内皮细胞增殖。

AIM： To investigate the effects of adrenomedullin （ADM） 2 （AM2） on proliferation of microvascular endothelial cells from the rat cerebral cortex. METHODS： Microvascular endothelial cells （MEC） were isolated from the cerebral cortex of SD rats and cultured. The cultured cells were identified using immunocylochemistry ashy with antibody for factor Ⅷ - related antigen and randomly distributed into eight experimental groups as follows： control, AM2 10^-7 mol/L, 10^-8mol/L, 10^-9 mol/L, ADM, ADM＋ AM2, 10% fetal bovine ..serum （FBS） stimulated, and 10% FBS＋ AM2 10^-7 mul/L groups. The proliferation of MEC was detected using [^3H ] -TdR incorporation assay. RESULTS： Compared with control, AM2（ 10^-7- 10^-9 mol/L）, ADM （10^-7 mol/L）, and AM2（10^-7mol/L） co- incubated with ADM （10^-7 mol/L） had no effects on [^3H] - TdR incorporation into the MEC （ P 〉 0.05）. 10% FBS induced [^3H] - TdR incorporation increased by 87.5% （ vs control, P 〈 0.05）, which was abolished by co - incubated the MEC with 10^-7 mol/L AM2（ P 〈 0.05）. CONCLUSION： AM2 inhibits FBS - stimulated proliferation of MEC from the rat cerebral cortex.