摘要
目的:观察急性多发脑梗死大鼠海马MARCKS mRNA表达的动态变化,探讨海马MARCKS基因表达动态变化与缺血损害的关系。方法:采用改良Kaneko法复制急性多发脑梗死模型,观察造模后大鼠的神经功能缺损评分改变,应用组织病理学方法观察模型1 h、6 h2、4 h7、2 h、7 d组缺血损害改变,并应用半定量PCR方法检测缺血各时点海马MARCKS mRNA表达水平。结果:造模后大鼠有不同程度的神经功能缺损表现,海马组织病理学改变以24 h后为著。急性缺血海马MARCKS mRNA均为过高表达,1 h开始升高,7 d最高,呈动态变化。结论:急性多发脑梗死大鼠海马MARCKS mRNA的表达异常升高,而MARCKS mRNA的过表达与海马缺血损害的发生密切相关。
AIM： To observe the dynamic alteration of myristoylated alanine - rich C kinase substrate （MARCKS） mR- NA expression in rat hippocampus with acute multi - cerebral infarction, and discuss the relationship between the alteration of hippocampus MARCKS gene and isehemia damage. METHODS： The acute multi - cerebral infarction model was established by method of Kaneko. Neurological function deficits were evaluated in the behavior test. The consequences of cerebral ischemic damage were examined by histopathological analyses. The MARCKS mRNA expression was measured by semi - quantitative PCR. RESULTS： The rats in acute multi - cerebral infaretion group showed different level changes of neurological function deficits. The hippocampus damage of histopathology became significant 24h after ischemia. At the same time, the MARCKS mRNA expression was upregulated at the area of rats hippocampus during ischemia, and its overexpression started lh after ischemia, and reached maximumTd after ischemia. CONCLUSION： MARCKS mRNA of rat hippocampus overexpresses during acute cerebral isehemia. Tiffs MARCKS mRNA overexpression is related with hippocampus ischemia damage.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2006年第5期964-967,共4页
Chinese Journal of Pathophysiology
基金
国家自然科学基金资助项目(No.30171196)
2003年教育部优秀青年教师基金
2005年教育部科学技术重点项目
