摘要
目的鉴定乌头碱在大鼠体内的主要代谢产物。方法灌胃给予雄性大鼠1.0mg/kg乌头碱后,收集24h尿液,固相萃取法提取,液相色谱-质谱法测定乌头碱及其代谢物。结果经与空白组对照发现给药后大鼠尿样中除乌头碱原体外还有4种代谢产物,并分别测得其准分子离子峰及其各级碎片离子。结论经与对照品比较及质谱断裂规律推断4个代谢产物分别为中乌头碱、16-O-去甲基乌头碱、16-O-去甲基中乌头碱、苯甲酰乌头碱。
Objective To determine the main metabolites of aconitine in urine in rats. Methods After oral administration of 1.0mg/kg aconitine, the urine of male rats was collected within 24h. The urine was extracted by solid phase extraction and analyzed by liquid chromatography - mass spectrometry. Results Aconitine and 4 metabolites were detected in the rat urine, and their protonate molecular ions and multistage fragment ions were monitored as compared with the blank control. Conclusion The metabolites were identi- fied as mesaconitine, 16 - o - demethylaconitine, 16 - o - demethylmesaconitine and benzoylaconitine, respectively.
出处
《中国法医学杂志》
CSCD
2006年第2期88-90,共3页
Chinese Journal of Forensic Medicine
