ω-3脂肪酸对人胃癌细胞凋亡信号传导的影响

The Effect of Omega 3 Fatty Acids on Signal Transduction for Apoptosis of the Human Gastric Cells

目的:观察EPA和DHA对人胃癌BGC-823细胞凋亡信号传导的影响。方法:采用MTT法测定肿瘤细胞抑制率,流式细胞检测肿瘤细胞凋亡率和bcl-2、Gα、PKCβⅡ基因蛋白的表达。结果:45μg/ml和48h时间点的EPA或DHA可显著抑制肿瘤细胞的生长(P<0.001),凋亡率明显升高(P<0.001),bcl-2基因蛋白表达下降(P<0.05),Gα和PKCβⅡ基因蛋白表达明显下降(P<0.001)。结论:ω-3脂肪酸可诱导人胃癌BGC-823细胞发生凋亡,信号传导途径Gα-PKCβⅡ的改变在细胞凋亡中起到重要的作用。

Objective： To assess the effects of EPA and DHA on signal transduction for apoptosis of the human gastric BGC-823 cells in vitro. Methods： Inhibitive rate of the cells was determined by MTT assay. Apoptotic rate and expression of bcl-2, Gα and PKCβⅡ proteins were determined using flow cytometry. Results： After treatment with 45μg/ml of EPA or DHA and at the exposed point of 48h, the growth of cells was markedly inhibited （P〈0.001）, and apoptotic rate was markedly increased （P〈 0.001）. Expression of bcl-2 was decreased （P〈0.05）, and expression of Gα and PKCβⅡ protein were markedly decreased （P〈0.001）. Conclusions： The ω-3 fatty acids can induce apoptosis of human gastric BGC-823 cells. Gα and PKCβⅡ signaling may play an important role in apoptosis of the cells.