白细胞介素18受体和CD30抗原在移植肾组织中的表达

Expression of interleukin 18 receptor and CD30 antigen in kidney transplant nephrectomy specimens

目的:探讨白细胞介素18受体(IL18R)和CD30抗原在移植肾排异反应中的局部表达及其临床意义。方法:采用链霉亲合素过氧化物酶(SP)免疫组织化学法检测44例移植肾切除标本[其中超急性排异反应(HAR)8例,急性排异反应(AR)16例,慢性排异反应(CR)12例和非免疫因素导致肾功能损害8例]及6例正常肾组织中IL18R和CD30的表达。结果:IL18R表达主要发生在肾小管上皮细胞,胞质表达为主。正常对照组肾小管上皮细胞表达为+～++,肾小球系膜细胞无表达。HAR、AR肾小管及肾小球的表达明显增强。CR组IL18R表达减弱。正常对照组肾小球、肾小管上皮细胞均无CD30抗原表达。HAR肾小球系膜细胞CD30(+),其余各组肾小球系膜细胞均无CD30抗原表达。CD30阳性均表现为胞质表达。肾小管上皮细胞CD30抗原表达多见于HAR和AR。CR及其他组肾小管上皮细胞CD30抗原表达较少。结论:IL18R和CD30抗原可能参与HAR、AR过程。检测IL18R和CD30抗原可为评价移植肾病理改变和肾移植预后提供重要参考。

Objective：To investigate the effects of Interleukin-18 receptor （IL-18R） and CD30 antigen in renal transplant allograft rejection. Methods： Paraffin-embedded kidney transplant nephrectomy specimens from 44 patients of allografted kidney, including eight with hyperacute rejection（HAR）, sixteen with acute rejection（AR）, twelve with chronic rejection（CR） and eight with damaged renal function by other factors, were conducted to examine IL-18R and CD30 antigen expression by ultra-sensitive S-P immunohistochemical staining . Results： IL-18R and CD30 antigen positive expression were mainly observed at the renal tubular epithelia in AR and HAR. The degree of IL-18R,CD30 antigen positive expression was associated with the degree of pathological damage in HAR and AR, and was significantly higher than normal control,CR and damaged kidney by nonimmunologic causes. Conclusions：IL-18R and CD30 antigen might participate in HAR and AR. Detection of IL-18R and CD30 antigen expression might be an important parameter to evaluate the pathological changes and prognosis in the renal allograft transplantation.