愈肝胶囊体外抗乙型肝炎病毒的作用

Inhibitory effect of Yugan capsule on hepatitis B virus in vitro

目的:通过观察愈肝胶囊对2．2．15细胞分泌的HBsAg、 HBeAg及HBV DNAN影响,探讨在体外水平上愈肝胶囊的抗乙肝病毒作用．方法:将实验家兔(n=6)随机分为3组:正常血清组、乙肝宁颗粒组、愈肝胶囊组,分别灌服洁净生理盐水5 mL／次,乙肝宁颗粒3．4 g／(kg·d),愈肝胶囊0．7 g／(kg·d),3次／d．测定含不同浓度愈肝胶囊血清抗HBV活性情况．2．2．15细胞培养上清液HBsAg、HBeAg测定采用ELISA法,HBV DNA检测采用 PCR杂交试验法．结果:作用5 d时,与乙肝宁颗粒组比较,含愈肝胶囊血清1:1 组抗HBV作用优于乙肝宁颗粒组(HBsAg:53．93±1．34 vs 41．03±0．85,P<0．05;HBeAg:55．25±1．42 vs 36．26±0．97, P<0．01;HBV DNA:56．81±2．37 vs 43．71±0．98．P<0．01); 1:2组、1:4组、1:8组差异无显著性;1:16组则作用较乙肝宁颗粒组低．药物作用10 d时,与细胞对照组及正常血清组比较,各含愈肝胶囊血清组抗HBV作用均比较显著．与乙肝宁颗粒组比较,含愈肝胶囊血清1:1组抗HBV DNA作用优于乙肝宁颗粒组(67．23±2．79 vs 48．02±1．03,P<0．05); 1:2组和1:4组对HBeAg及HBV DNA的作用优于乙肝宁颗粒组;1:8组和1:16组对HBV DNA作用低于乙肝宁颗粒组．结论:含愈肝胶囊血清在体外细胞培养中对2．2．15细胞分泌 HBsAg、HBeAg及HBV DNA均有较好的抑制作用,其抑制作用随其浓度增大而增强．

AIM： To investigate the anti-hepatitis B virus effect of Yugan capsule in vitro. METHODS： The experimental rabbits （n = 6） were randomly divided into 3 groups （2 for each）, and then were treated with the normal serum, Yiganning granule and Yugan capsule, respectively. The blood samples were collected from the hearts and diluted into various concentrations. The effects of the serums on the 2.2.15 cell line were observed. Enzyme linked immunosorbent assay was used to detect the the contents of HBsAg and HBeAg, and polymerase chain reaction and hybridization were used to determine the contents of HBV DNA. RESULTS： Five days after treatment, Yugan capsule of 1 ： 1 group had significant anti-HBV effect in comparison with Yiganning granule （HBsAg： 53.93±1.34 vs 41.03±0.85, P 〈0.05; HBeAg： 55.25±1.42 vs 36.26±0.97, P 〈0.01; HBV DNA： 56.81±2.37 vs 43.71±0.98, P 〈0.01）, while Yugan capsule of 1 ： 2, 1 ： 4, and 1 ： 8 group was not markedly different, and that of 1 ： 16 group had less anti-HBV effect. Ten days after treatment, Yugan capsule of different concentrations inhibited the proliferation of HBV significantly in comparison with cell control and normal serum group, while in comparison with Yiganning granule, Yugan capsule of 1 ： 1 group notably inhibited the replication of HBV DNA （67.23±2.79 vs 48.02±1.03, P 〈0.05）. Also Yugan capsule of 1 ： 2 and 1 ： 4 group was superior to Yiganning granule in inhibiting the secretion of HBeAg and replication of HBV DNA, but that of 1 ： 8 and 1 ： 16 group was inferior to Yiganning. CONCLUSION： Yugan capsule serum can inhibit the secretion of HBsAg, HBeAg, and replication of HBV DNA in 2.2.15 cells cultured in vitro in a concentration-depended manner.