胰腺癌组织中SKP2和P27蛋白表达及其相互关系

Expression and correlations of S-phase kinase associated protein 2 and P27 protein in pancreatic cancer tissues: an analyses of 51 cases

目的:研究胰腺导管癌和慢性胰腺炎组织中(S期激酶相关蛋白-2)SKP2和P27表达,探讨其临床病理意义及两者在胰腺导管癌中表达的相互关系．方法:51例胰腺导管癌和10例慢性胰腺炎手术切除标本常规制作石蜡包埋切片,SKP2和P27染色方法为SP免疫组化法．结果:51例胰腺导管癌SKP2阳性表达28例(54．9％)和P27阳性表达25例(49．0％),10例慢性胰腺炎SKP2阳性2例(20．0％) 和P27阳性9例(90．0％),两者之间均存在显著差异(P<0．05), 高分化腺癌(7／20,35．0％)和未转移(5／16,31.2％)病例SKP2表达阳性率明显低于低分化腺癌(14／19,73．7％)和转移(23／35, 65．7％)病例,均存在显著差异(P<0．05);高分化腺癌(13／20, 65．0％)和未转移(12／16,75．0％)病例P27表达阳性率明显高于低分化腺癌(6／19,31．5％)和转移(13／35,37．1％)病例,有显著或高度显著差异(P<0．05或P<0．01)．SKP2和P27在胰腺导管癌中表达呈密切相关(x2=14．33,P<0．01)．结论:SKP2和P27表达是反映胰腺导管癌发生,发展及预后的重要生物学标记物,SKP2阳性表达或P27阴性表达者恶性度高、易发生转移及预后不良,且两者表达存在相互调控作用．

AIM： To detect the expression of S-phase kinase associated protein 2 （SKP2） and P27 protein in human pancreatic ductal carcinoma and chronic pancreatitis, and to investigate the clinical significance and their correlations in the pancreatic ductal carcinoma. METHODS： SP immunohistochemical method was used to detect the expression of SKP2 and P27 in the routinely paraffin-embedded sections of specimens from patients with pancreatic ductal carcinoma （n = 51） and chronic pancreatitis （n = 10）. RESULTS： The positive rate of SKP2 expression in the pancreatic ductal carcinoma （28/51, 54.9%） was significantly higher than that in the chronic pancreatitis （2/10, 20.0%, P 〈 0.05）, while the rate of P27 was significantly lower [25/51（49.0%） vs 9/10（90.0%）, P 〈 0.05]. The positive rates of SKP2 expression was significantly lower in the well-differentiated （7/20, 35.0%） and non-metastasis cases （5/16, 31.2%） than those in the poorly-differentiated （14/19, 73.7%）and metastasis ones （23/35, 65.7%） （P 〈 0.05）, while the rate of P27 expression was significantly higher in the well-differentiated （13/20, 65.0%） and non-metastasis cases （12/16, 75.0%） than those in the poorly-differentiated （6/19, 31.5%） and metastasis ones （13/35, 37.1%） （P 〈 0.05 or P 〈 0.01）. The expression of SKP2 and P27 were closely correlated in the pancreatic ductal carcinoma tissue （χ^2=14.33, P〈0.01）. CONCLUSION： SKP2 and P27 are important biological markers for reflecting the carcinogenesis, progression, and prognosis of pancreatic ductal carcinoma. The positive expression of SKP2 or the negative expression of P27 reveals more serious status of the illness, the tendency of metastasis and unfavorable prognosis. There may be a co-regulatory relationship between SKP2 and P27 expression.