胰岛素样生长因子对早期胚胎发育的保护作用

Protective effect of insulin-like growth factors on early pregnancy

目的:探讨胰岛素样生长因子I和II(IGFI和II)在正常胚胎发育及早期胚胎发育终止绒毛及蜕膜组织中的表达及其在母体血中水平的改变与胚胎发育的关系.方法:分别采用免疫组织化学SP法和原位杂交法检测正常胚胎发育绒毛及早期胚胎发育终止绒毛中IGFI和蜕膜中IGFII蛋白的表达以及IGFImRNA和蜕膜中IGFIImRNA的表达;采用酶联免疫吸附试验(ELISA)检测正常妊娠及早期胚胎发育终止孕妇血中IGFI和II水平的变化.结果:免疫组化结果显示,正常妊娠绒毛中IGFI和蜕膜中IGFII的表达明显高于胚胎发育终止组(P<0.01);原位杂交结果显示,正常妊娠组绒毛中IGFI和蜕膜中IGFIImRNA的表达明显高于胚胎发育终止组(P<0.01);正常妊娠组母血中IGFI和II水平显著高于胚胎发育终止组(P<0.01).结论:IGFI和II在绒毛组织中的表达及母血中的水平的升高对早期胚胎发育有重要的保护作用,其含量的减少可能是导致胚胎死亡的重要原因之一.IGFI和II可能成为预测胎儿安危的指标之一.

AIM： To detect the expressions of insulin-like growth factor- Ⅰ （IGF-Ⅰ） in villus and IGF-Ⅱ in decidua and the levels in mother serum, and to explore their relationship with the embryogenesis of early pregnancy. METHODS： Expressions of IGF-Ⅰ and IGF-Ⅱ were examined by immunohistochemical SP method; the mRNA expressions of IGF-Ⅰ and IGF-Ⅱ were detected by in situ hybridization （ISH） ; serum IGF-Ⅰ and IGF-Ⅱ levels were determined by enzyme-linked immunosorbent assay （ELISA）. RESULTS： The expressions of IGF-Ⅰ and IGF-Ⅱ both protein and mRNA were lower in the abnormal villus and decidua of pregnancy termination than those of normal villus and decidua of normal pregnancy （ P 〈0.01 ）. Serum levels of IGF-Ⅰ and IGF-Ⅱ in normal pregnancy women were much higher than those of abnormal pregnancy women （P 〈 0.01 ）. CONCLUSION： IGF-Ⅰ and IGF-Ⅱ may play important roles in the regulation of fetal growth, the decrease of IGF-Ⅰ and IGF-Ⅱ levels in villus, decidua and serum may be the key factor contributing to the embryogenesis termination of early pregnancy. So IGF- Ⅰ and IGF-Ⅱ can serve as 2 parameters in examining the fetal safety.