脊髓爆震伤后早期脊髓前角运动神经元的形态学变化

Early morphological changes of ante- rior horn motor neurons after explosive injury of spinal cord

目的:建立脊髓爆震伤动物实验模型,探讨脊髓爆震伤后早期脊髓前角运动神经元形态学变化.方法:将36只家兔随机分为对照组(A组,n=12),6h实验组(B组,n=12)及24h实验组(C组,n=12),采用0.9g单质金属炸药黑索金(RDX)将B,C组家兔炸伤,分别于伤后6h及24h两个时间点取材,进行HE染色、甲苯胺蓝染色及银浸染色,观察脊髓神经元形态学改变.结果:脊髓爆震伤后6h脊髓运动神经元发生可逆性改变,部分神经元坏死,死亡均数为12.58±2.23,而伤后24h脊髓运动神经元大量坏死,死亡均数达到了31.52±2.69,与对照组相比差异有统计学意义(P<0.01).结论:脊髓爆震伤后6h内脊髓运动神经元以可逆性改变为主,提出早期发现和治疗的重要性.

AIM： To establish an animal model with explosive injury of spinal cord, and to explore the early morphological changes of anterior horn motor neurons after explosive injury of spinal cord. METHODS： Thirty-six rabbits were randomly divided into control group （Group A, n = 12）, 6 h experimental group （ Group B, n = 12） and 24 h experimental group （ Group C, n = 12）. Group B and C were injured with 0.9 g cyclotrimethylene trinitramine, and 6 h and 24 h after trauma, the injured spinal cords were taken for observation of the morphological changes and apoptosis of spinal motor neurons using HE staining, toluidine blue staining and Cajal staining under light microscopes. RESULTS： The reversible change of the neurons of the rabbits happened after 6 h, and some neurons was observed dead, the mean of the death being 12.58 ±2.23; 24 h after trauma, a great quantity of neurons were observed dead, the mean of the death being 31.52 ±2.69, which differed significantly from that in the control group （P 〈0.01）. CONCLUSION： Most of the damage is reversible within 6 h after trauma,therefore the early discovery and treatment should be emphasized.