贴敷“咳喘宁”贴膏对支气管哮喘模型大鼠肺泡灌洗液中炎性细胞的影响

The influence of Kechuanning plaster applying on the points to airway inflammation in bronchoalveolar lavage fluid on airway inflammation of rats with asthma

目的:通过建立大鼠支气管哮喘模型,观察穴位贴敷“咳喘宁”贴膏对支气管哮喘模型大鼠肺泡灌洗液中炎性细胞的影响。方法:将60只Wistar大鼠按随机数字表分组法随机分为空白对照组(A组)、模型对照组(B组)、内服“桂龙咳喘宁”对照组(C组)、内服“地塞米松”对照组(D组)、穴位贴敷“咳喘宁”高剂量治疗组(E组)、穴位贴敷“咳喘宁”低剂量治疗组(F组)6组,每组10只。各组大鼠用Elwood法造模。于诱喘前10h开始给药,并分别观察各组哮喘模型大鼠肺泡灌洗液(BALF)中炎性细胞的改变。结果:与C组比较,D、E、F组炎性细胞减少有显著性差异(P<0.05);与D组比较,C组、E组、F组嗜酸性粒细胞明显增高,有显著性差异(P<0.05);各治疗组中的中性粒细胞都明显减少,与B组比较有显著性差异(P<0.05);各治疗组中淋巴细胞水平明显降低,P<0.05或P<0.01。结论:穴位贴敷“咳喘宁”贴膏对支气管哮喘模型大鼠有明显的抑制炎症的作用,其作用虽不及内服“地塞米松”组,但等同内服“桂龙咳喘宁”组;有明显的改善变态反应和增加免疫功能作用。

Objective：Observed the influence of Keehuanning piaster applying on the points to airway inflammation in bronchoalveolar lavage fluid on airway inflammation of rats with asthma. Methods： 60 rats were randomly divided into 6 groups： normal group（A group）,model group （B group）,Guilong Kechuanning group（C group）, Dexamethasone group （D group）, Kechuanning high group （E group） and Kechuanning low group （F group）. And each group had 10 rats. The model of asthma was established by Elwood ways. Each group was given corresponding treatment before inhale ovalbumin 10 hours.Then observed the change of the number of inflammatory cells in bronchoalveolar lavage fluid. Results： After treatment, the number of inflammatory ceils in bronchoalveolar lavage fluid in D, E and F group turned to higher than that of C group（P 〈 0.05）, but E and F group didn＇t show the same effect with D （P 〉 0.05）. Conclusion：Kechuanning plaster applying on the points can restrain airway inflammation of rats with asthma.Kechuanning plaster and Dexamethasone have approximately effect on restrain airway inflammation of rats with asthma, and better than Guilong Kechuanning.